Genetic characterization of varicella-zoster virus in people aged 20 years and under in Yichang City of Hubei Province, 2019-2020 / 中华流行病学杂志

Objective: To analyze the genetic characteristics of varicella-zoster virus (VZV) in people aged 20 years and under in Yichang City of Hubei Province from 2019 to 2020. Methods: Based on the Yichang Health Big Data Platform, we investigated cases 20 and under clinically diagnosed as herpes zoster in three hospitals from March 2019 to September 2020. Collecting vesicle fluid and throat swab samples of the cases and completing questionnaires to obtain basic information. Real-time fluorescent quantitative PCR was used for positive identification of the virus. PCR amplification of VZV's open reading frame (ORF) and sequencing of the products to determine the VZV genotype. Analyze mutations at some specific single nucleotide polymorphism (SNP) sites. Results: Among 46 cases of herpes zoster, the male to female ratio was 1.3∶1 (26∶20) and the age ranged from 7 to 20 years old. Fifteen cases had been vaccinated against varicella, including 13 and 2 cases of 1 and 2 doses, respectively. VZV strains were detected in 34 samples (73.91%), all belonging to Clade 2. Phylogenetic tree analysis of the nucleotide of ORF22 showed, compared with Clade 2 referenced strains, the sequence matching degree of nucleotide for all 34 samples was 99.0% to 100.0%. Conclusion: The main VZV strain causing herpes zoster in people aged 20 years and under in Yichang from 2019 to 2020 was Clade 2.

Effect of the varicella vaccination on the clinical characteristics of herpes zoster cases aged 20 years and under / 中华预防医学杂志

To discuss the effect of varicella vaccination on the clinical characteristics of herpes zoster (shingles) cases aged 20 years and under, and analyze its clinical features. Based on the Yichang Health Big Data Platform, a descriptive study was conducted to collect the information of cases aged 20 years and under in three medical institutions of Yichang Central People's Hospital, Yichang First People's Hospital and Yichang Second People's Hospital from March 2019 to September 2020. According to the history of varicella vaccine, cases were divided into vaccination group and non-vaccination group, and their clinical features and outcomes were compared. The results showed that 46 shingles cases, aged from 7 to 20 years old, were included in this study. 26 males (56.5%), 20 females (43.5%), 15 cases in vaccination group (32.6%) and 31 cases in non-vaccination group (67.4%). 28 cases had thoracic involvement, followed by lumbar (n=8), cranial (n=7) involvements and extremities (n=7). The spread of herpes skin area: 2 cases involved too large area, 21 cases of 10 cm×10 cm, 14 cases of 5 cm×5 cm, 9 cases of 1 cm×1 cm. Herpes number: 26 cases had 10-49 herpes, followed by <10 herpes (n=9), uncountable herpes (n=7) and 50-99 herpes (n=4). The clinical course[M(Q1,Q3)] lasted 20.5 (13.5,24.8) d averagely, 5 cases had postherpetic neuralgia (PHN) and 1 case had respiratory complications. Shingles decrustation time was significantly shorter in vaccination group (Z=-2.01, P<0.05), and there was no significant difference in other characteristics by vaccination. In conclusion, the number and spread of shingles in most children and adolescents are less, and the complications such as PHN are less. Varicella vaccination can reduce the decrustation time and relieve shingles cases with some clinical symptoms.

Effects of Toluene on the Development of the Inner Ear and Lateral Line Sensory System of Zebrafish / 生物医学与环境科学（英文）

Objective@#The aim of this study was to explore the ototoxicity of toluene in the early development of zebrafish embryos/larvae.@*Methods@#Zebrafish were utilized to explore the ototoxicity of toluene. Locomotion analysis, immunofluorescence, and qPCR were used to understand the phenotypes and molecular mechanisms of toluene ototoxicity.@*Results@#The results demonstrated that at 2 mmol/L, toluene induced zebrafish larvae death at 120 hours post fertilization (hpf) at a rate of 25.79% and inhibited the rate of hatching at 72 hpf. Furthermore, toluene exposure inhibited the distance travelled and average swimming velocity of zebrafish larvae while increasing the frequency of movements. As shown by fluorescence staining of hair cells, toluene inhibited the formation of lateral line neuromasts and middle line 1 (Ml @*Conclusion@#This study indicated that toluene may affect the development of both the inner ear and lateral line systems in zebrafish, while the lateral line system may be more sensitive to toluene than the inner ear.

Quantitative assessment of occupational health risks in workers exposed to low-level dichloromethane / 中国职业医学

OBJECTIVE: To quantitatively evaluate the non-carcinogenic and carcinogenic risks of low-level dichloromethane(DCM) exposure. METHODS: A typical sampling method was used to select workers from an engine factory and an automobile parts factory as the research subjects. After hazard identification and hazard characteristic assessment, the occupational health risks of DCM in the workplace were quantitatively assessed by non-carcinogenic risk assessment and carcinogenic risk assessment. RESULTS: The highest exposure concentration of short-term of DCM was 43.6 mg/m~3 in the air of eight DCM job posts in these two enterprises, which was lower than the operational level of 300.0 mg/m~3(half of the occupational exposure limit in China). Under this premise, the non-carcinogenic risk assessment results showed that the non-carcinogenic risk caused by inhalation of DCM was unacceptable in workers of these eight job posts. The results of carcinogenic risk assessment showed that the carcinogenic risk caused by inhalation of DCM was acceptable for all the workers in these eight job posts. CONCLUSION: Low-level DCM exposure can cause unacceptable occupational health risks to workers. Quantitative risk assessment can be applied to assess exposure to low-level chemicals.

Evaluating the effect of intervention on the use of respiratory protective equipment among migrant workers / 中国职业医学

OBJECTIVE: To explore the effect of intervention adherence on the use of respiratory protective equipment(RPE) among migrant workers who occupationally exposed to organic solvents. METHODS: A total of 901 migrant workers from 60 small and medium-sized enterprises exposed to organic solvents in a district of Guangzhou City were selected as study subjects using three-arm cluster randomized controlled trial. The enterprises were divided into control group, intervention group, and combined intervention group. Migrant workers in the control group did not receive any intervention, while migrant workers in the intervention group received traditional health education and mobile health(mHealth) intervention, and migrant workers in the combined intervention group received interventions of the intervention group plus peer education. The use of RPE by the migrant workers was evaluated in these three groups in the last week, and at the sixth month. RESULTS: The adherence rates of the three mHealth intervention measures(following the WeChat official account of the project, joining the WeChat/QQ group of the project, and reading the provided messages from project team) in the combined intervention group were higher than that in the intervention group(74.8% vs 53.6%, 70.7% vs 41.8%, 78.5% vs 65.0%, all P<0.01). In the two intervention groups, the adherence rates of traditional health education(attending occupational health training, and browsing public welfare posters) were higher than that of mHealth intervention and/or peer education(all P<0.01). Logistic regression analysis showed that regardless of mHealth intervention, traditional health education or peer education, the high compliance subgroups of the two intervention groups were more likely to wear RPE all the time(all P<0.05) compared with the control group. The intervention effectiveness of the combined intervention group was better than that of the intervention group. CONCLUSION: Improving compliance with occupational health education interventions can promote the use of RPE among migrant workers. The implementation of peer education is beneficial to improve intervention compliance and intervention effect of migrant workers.

A Study on the Effects of Occlusal Function on CTGF Expression in Orthodontics / 昆明医科大学学报

Objective To investigate CTGF changes in the expression of the gum tissue before and after orthodontic treatment, and to preliminarily explore the modification mechanism of gingival tissue and the effectiveness of the intervention measures. Methods 72 male Sprague-Dawley rats of 12-week-old, weight about 250 g-300 g, randomly divided into 6 groups: blank control group (A), healthy teeth orthodontic group (B), low functional group (C), low occlusal function teeth orthodontic group (D), combined intervention group (E), bite orthodontic intervention group (F). The results of the study were compared at 1 w, 2 w, 4 w, 6 w.Results (1) HE staining results showed the atrophy of the gingival tissue, which suggested that occlusal hypofunction SD rats model were successfully established. (2) Fluorescence quantitative results of CTGF in gingival tissue: 6 w: group B was higher than group D and group F (P＜0.05). Conclusion (1) the expression of CTGF in the low occlusion group was lower than that of the normal control group with time, and the gum tissue was vulnerable to atrophy. (2) It remains to be further studied whether the bite force recovery is effective.

Minimally invasive conjunctivorhinostomy for the treatment of complete bicanalicular obstruction / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To investigate the effectiveness of minimally invasive conjunctivorhinostomy for complete bicanalicular obstruction, and to evaluate the advantages, complications, and degree of satisfaction after this technique. ●METHODS: Twenty - two patients ( 22 eyes ) with complete bicanalicular obstruction were selected as the research subjects, all patients were treated by minimally invasive conjunctivorhinostomy. The post - operation follow- up lasted for 3 - 27mo. Medical records were reviewed for demographic data, surgical time, length of the tubes, duration of follow-up, patency of the tube, postoperative complications, whether satisfied or dissatisfied with this procedure. Meanwhile, the data was analyzed for evaluating the clinical efficacy of minimally invasive conjunctivorhinostomy. ●RESULTS: The overall operative success rate was 95%(21/22), and the overall patient satisfaction was 68% (15/22 ) . The post - operation complications included:granulation tissue proliferation of the lower end of the tube 2 cases (9%), middle turbinate blocked the opening of tube 3 cases ( 14%) , nasal mucosa covering the opening of tube 1 case ( 5%) , the tube was blocked by secreta 5 cases ( 23%) , foreign body sensation 10 cases (45%), dry eye 2 cases ( 9%), regurgitation of nasal secreta discharge 8 cases (36%). ●CONCLUSION: Minimally invasive conjunctivorhinostomy can treat the complete bicanalicular obstruction. This operation method is characterized by simplicity, high success rate, short operative time, less tissue trauma, less complications, without facial scarring, and it is worth clinical promotion.

Expression of Gli1 in Adult Acute Lymphoblastic Leukemia and Its Clinical Significance / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To explore the expression and clinical significance of Hedgehog signaling transcription factor Gli1 in acute lymphoblastic leukemia (ALL) patients.</p><p><b>METHODS</b>The clinical specimens were obtained from 32 newly diagnosed and 6 relapsed ALL patients. Normal bone marrow cells from 15 healthy donors were used as controls. Real-time qPCR and Western blot were applied to detect Gli1 mRNA and protein expression in bone marrow mononuclear cells (BMMNC) of these samples respectively. The relation of Gli1 mRNA levels with clinical parameter was also evaluated.</p><p><b>RESULTS</b>The expression level of Gli1 mRNA in de novo and relapsed ALL patients was significantly higher than that in the normal controls (P < 0.05). There was no stalistically significant difference of the Gli1 mRNA expression between de novo and relapsed ALL cases (P > 0.05). In 24 de novo ALL patients with complete remission (CR) after induction chemotherapy, the levels of Gli1 mRNA were significantly reduced as compared with levels before treatment (P < 0.05). However, in 4 ALL patients without remission, no obvious difference of Gli1 mRNA levels were observed as compared with levels of Gli1 before treatment (P > 0.05). A positive correlation between the Gli1 mRNA expression level and white blood cell count (WBC) was found in the BMMNC of ALL patients (R = 0.725, P < 0.05). Similarly, Gli1 protein expression was significantly higher in the de novo and relapsed ALL cases compared with normal controls. The Gli1 protein level was down-regulated when the ALL patients was in CR.</p><p><b>CONCLUSION</b>The expression of Gli1 mRNA and protein has been found to be high in de novo and relapsed ALL patients, and the change of Gli1 expression maybe relate to therapeutic efficacy and prognosis of ALL patients.</p>

Application of electric detachable stent in the embolization therapy of intracranial aneurysms / 中华外科杂志

<p><b>OBJECTIVE</b>To evaluate the efficacy and safety of Solitaire(TM) AB neurovascular stenting-assisted coil embolization for patients with wide-necked or dissecting aneurysms.</p><p><b>METHODS</b>The clinical results and prognosis from a consecutive series of 38 patients with 40 wide-necked or dissecting aneurysms aneurysms who treated by Solitaire(TM) AB neurovascular stenting-assisted coil embolization from August 2010 to January 2012 was retrospectively analyzed. There were 12 male and 26 female patients, the age was 21 - 78 years (mean 55 years). Thirty-one cases were confirmed wide-neck aneurysms and 9 cases were dissection aneurysms by DSA. Acute subarachnoed hemorrhage due to the rupture of aneurysms was seen in 28 cases (according Hunt-Hess scale, 1 case of Class I, 20 cases of Class II, 4 cases of Class III, 3 cases of Class IV), 1 case was traumatic intracranial aneurysm, 1 case was misdiagnosed during the operation of pituitary adenoma by the approach of transsphenoid, and unruptured aneurysms were seen in 8 cases. The aneurysms were located at the posterior communicating segment of internal carotid artery (21 cases), the supraclinoid segment of internal carotid artery (6 cases), the cavernous segment of internal carotid artery (3 cases), the anterior communicating artery (1 case), and the vertebral artery (9 cases). The patients were performed DSA and Glasgow outcome score (GOS) to evaluate the prognosis 6 months after surgery.</p><p><b>RESULTS</b>Forty stents were used and all remodeling device were achieved successful position. Owing to acute thrombosis in 3 patients, the stents were retrieved successfully. The proportion of patients in whom Raymond class 1 occlusion was obtained in 31 cases (77.5%), Raymond class 2 occlusion in 5 cases (12.5%) and Raymond class 3 occlusion in 4 cases (10.0%). The follow-up was 3 to 12 months (median 6 months). The results of DSA indicated none of the patients' anuerysm was recurred; and GOS was applied to evaluate the prognosis of patients after 3 months. Of 38 patients, 34 recovered well, 3 moderately disabled, 1 patient died.</p><p><b>CONCLUSIONS</b>It is safe to embolize aneurysms with Solitaire(TM) AB neurovascular stenting-assisted coil; meanwhile, the stents can be retrieved when acute thrombosis to reduce the complications.</p>

Effects of noise exposure level on the relationship between SNPs of SOD1 and the susceptibility to noise-induced hearing loss (NIHL) / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the effects of noise exposure level and cumulative noise exposure (CNE) on the relationship between rs2070424 and rs10432782 SNPs in SOD1 and the susceptibility to noise-induced hearing loss (NIHL).</p><p><b>METHODS</b>A case-control study was performed for investigating the effects of environmental risk factors on the susceptibility to NIHL in 201 sensitive workers and 202 resistant workers.A questionnaire was utilized to investigate the occupational health and to identify the occupational risk factors. The noise exposure levels were detected according to the Chinese standard Measurement of noise in the workplace (GBZ/T 189.8-2007). The peripheral blood samples (5 ml blood for each sample) were from sensitive workers and resistant workers. Genomic DNA was extracted on the basis of the standard procedures of Takara kit. SNPs were detected using standard procedures of TaqMan probe allele identification method.</p><p><b>RESULTS</b>In group exposed to 85 - 92 dB noise (A), the risk of NIHL in the subjects with the AA genotype of rs2070424 was lower than that in the subjects with the GG genotype, OR = 0.37 (95%CI: 0.17∼ 0.80). In group exposed to > 82 dB CNE (A), the AA genotype of rs2070424 is a protective factor of NIHL, as compared with the GG genotype, OR = 0.25 (95%CI: 0.09 ∼ 0.70). In group exposed to 85 - 92 dB noise (A), the risk of NIHL in the subjects with the GG genotype of rs10432782 was compared with the risk of NIHL in the subjects with the TT genotype, OR = 3.17 (95%CI: 1.16 ∼ 6.89). The GT genotype was compared with TT genotype, OR = 2.39 (95%CI: 1.16 ∼ 4.97). In group exposed to 75 ∼ 82 dB CNE (A), the risk of NIHL in the subjects with the GG genotype was compared with the risk of NIHL in the subjects with the TT genotype, OR = 2.35 (95%CI: 0.96 ∼ 5.72), P = 0.06. The GG genotype may bea risk factor of NIHJ.</p><p><b>CONCLUSION</b>The noise exposure level and CNE may influence the relationship between rs2070424, rs10432782 SNPs in SOD1 and noise-induced hearing loss.</p>

Efficacy of compound xuanju capsule on type-III prostatitis-related sexual dysfunction / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the value of Compound Xuanju Capsule in the treatment of type-III prostatitis-related sexual dysfunction.</p><p><b>METHODS</b>We randomly divided 90 type-III prostatitis patients with sexual dysfunction diagnosed by NIH clinical criteria into an experiment group and a control group to be treated with Compound Xuanju Capsule and antibiotics, respectively. We analyzed the therapeutic results based on the scores on chronic prostatitis symptom index (CPSI), prostatitis-related sexual function index (PSFI ) and self-rating anxiety scale (SAS), and compared them between the two groups and with the baseline data.</p><p><b>RESULTS</b>The degree of prostatitis-related sexual dysfunction was not correlated with that of prostatitis symptoms. Prostatitis symptoms and sexual function were significantly improved in the experiment group than in the control (P < 0.05), and the SAS score was markedly lower in the former than in the latter (P < 0.05).</p><p><b>CONCLUSION</b>Compound Xuanju Capsule can not only alleviate the symptoms of type-III prostatitis, but also improve its related sexual dysfunction and anxiety.</p>

The effects of oxidative damage induced by organic oxidant t-BHP on cochlear hair cells / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To establish the oxidative damage model of cochlea hair cells using organic oxidant t-BHP in vitro.</p><p><b>METHODS</b>HEI-OC1 cells were exposed to t-BHP at 8 doses (30~4000 µmol/L) for 12 h. Trypan blue test was used to detected the cellular viability and MTT assay was utilized to measured the cellular proliferation. The intracellular ROS levels were determined by 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA).</p><p><b>RESULTS</b>The survival rates of HEI-OC1 cells started decrease significantly at the dose of 100 µmol/L t-BHP, the peak of decreased survival rates appeared at the doses of 200~800 µmol/L. The results of MTT assay demonstrated that 30 µmol/L t-BHP could promote cellular proliferation ability, when t-BHP concentrations were higher than 200 µmol/L, the cellular proliferation ability was inhibited. The results of DCFH-DA assay showed that there was no fluorescence in control group, the strong fluorescence was observed in positive control group, the weak fluorescence was observed in 30~50 µmol/L t-BHP groups, the bright fluorescence was observed in 100 µmol/L t-BHP group, still the stronger fluorescence was observed in 200~1000 µmol/L groups, but the cellular number decreased with the doses because of the lower cellular viability.</p><p><b>CONCLUSION</b>The exposure to 100 µmol/L t-BHP for 12 h could simulate the oxidative damage induced by noise in cochlear hair cells.</p>

The efficacy of collagen-hydroxyapatite composite membrane on bone regeneration / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the efficacy of a new collagen-hydroxyapatite (COL-HA) composite membrane on bone regeneration of SD rat cranial defects.</p><p><b>METHODS</b>Four defects were produced in the calvaria of 24 SD rats. The animals were divided into four groups: Empty defects without membrane (group 1); defects covered by COL-HA single-layer dense membranes (group 2); defects covered by COL-HA double-layer membranes (group 3); defects covered by Bio-Gide membranes (group 4). At 2, 4, 8 and 12 weeks after surgery, 6 rats were sacrificed and the following parameters were analyzed: Macroscopic observation, X-ray examination, descriptive histology, regenerate bone quantitative histology. Statistical analysis consisted of generalized linear models/factorial design analysis of variance and LSD-t test was performed.</p><p><b>RESULTS</b>Since two weeks after surgery, there were a small amount of bone regenerated in three groups except group 1. At 12 weeks after surgery, the opaque sclerous tissues filled with the defects in three groups, and residual membrane fragments still could be found. X-ray pictures showed the density of regenerate bone in group 3 and group 4 was closed to the original bone and greater than that of group 2. Quantitative analysis of regenerate bone showed that in initial stage, group 4 had more bone regeneration than the other groups (P < 0.05), and at 12 weeks after surgery the differences between group 4 and group 2/group 3 had no statistically significant.</p><p><b>CONCLUSION</b>The COL-HA composite membranes can guide bone regeneration of rat cranial defects. The efficacy of bone regeneration of COL-HA double-layer membrane is superior to COL-HA single-layer dense membrane, because its property is more propitious to the adherence and proliferation of osteoblasts.</p>

Association between SNPs in SOD1 and noise-induced hearing loss in Chinese Han population / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To observe the association between SNPs in SOD1 (rs1041740, rs2070424, rs10432782 and rs4998557) and noise-induced hearing loss in Chinese Han population.</p><p><b>METHODS</b>A case-control study was used to study the effects of environmental risk factors on the susceptibility to noise induced hearing loss (NIHL) in 201 sensitive workers and 202 resistant workers. A questionnaire was designed to carry out an investigation, and an occupational health survey was used to identify the occupational risk factors. Genomic DNA was extracted from peripheral blood cells using standard procedures of Takara kit, and 5 ml blood was from each subject. SNPs were detected using standard procedures of TaqMan probe allele identification method.</p><p><b>RESULTS</b>In SOD1 gene, the A allele of rs2070424 was a protective factor of NIHL, compared with the G allele (OR = 0.67, 95%CI: 0.50 approximately 0.88). The risk with NIHL in subjects with AA genotype was significantly lower than that in subjects with GG genotype (OR = 0.48, 95%CI: 0.26 approximately 0.79). After adjusting the confusion factors, OR was 0.44 and 95%CI was 0.25 approximately 0.78.</p><p><b>CONCLUSION</b>In Chinese Han population, the SNP of rs2070424 in SOD1 may be associated with the susceptibility to NIHL.</p>

Effects of SOD2 and its C47T mutation on oxidative injury in cochlea hair cell / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the effect of SOD2 and its C47T mutation on oxidative injury in cochlea hair cells.</p><p><b>METHODS</b>HEI-OC1 cells were transfected with the SOD2 of Ala16 and Vla16. Cells' proliferation ability was determined by MTT assay. The intracellular SOD2 activities were detected by xanthine oxidase method. Intracellular ROS were determined by DCFH-DA after exposure to 100 µmol/L t-BHP and the early apoptotic and necrotic rate or late apoptotic rate were quantified by flow cytometry (FCM) using Annexin V/PI double staining.</p><p><b>RESULTS</b>MTT method showed the transfection of SOD2 gene and empty plasmid did not affect the proliferation capacity. SOD2 vitality in Ala(16) and Val(16) SOD2 transfected cells increased 2.51 and 2.71 times respectively (P < 0.01), but the difference between the two transfection groups was not statistically significant (P > 0.05). After exposed to t-BHP, the majority of the untransfected and empty plasmid transfected cells sent '++' class bright fluorescence, while in Ala(16) and Val(16) SOD2 transfected groups, only about half cells sent '±' ∼ '+' level fuzzy fluorescence. determination of FCM suggested the early apoptotic and necrotic rate or late apoptotic rate decreased after SOD2 transfection (P < 0.01), but the difference between the two genotypes of SOD2 was not statistically significant (P > 0.05).</p><p><b>CONCLUSION</b>High expression of SOD2 below 3.71 times can reduce intracellular ROS level in HEI-OC1 cells, while SOD2 C47T mutation had no effect on them. SOD2 can be considered as NIHL susceptibility gene and its rs4880 SNP may be not directly related to NIHL genetic susceptibility.</p>

Effect of phosphatidylinositol 3-kinase/serine-threonine kinase signalling pathway during the proliferation process of HL-60 cells exposed to benzoquinone / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the effects of phosphatidylinositol 3-kinase/Serine-threonine kinase (PI3K/Akt) signal pathway on the proliferation of HL-60 cells exposed to benzoquinone (BQ).</p><p><b>METHODS</b>HL60 cells were divided into 3 groups: control group (treated with PBS), BQ group (treated with 3 micromol/L BQ) and LY294002 plus BQ group (treated with 20 micromol/L LY294002 plus 3 micromol/L BQ). The cell proliferation was measured with alamar blue dye assay. Western blot assay was used to detect the expression of p-Akt and Akt proteins and flow cytometer was used to observe the cell cycle.</p><p><b>RESULTS</b>The cell proliferation rate and the cell proportion in the S, G2 phase of BQ group were 185.00% +/- 30.00%, 48.23% +/- 1.37% and 15.40% +/- 1.21%, respectively, which were significantly higher than those (100.00% +/- 0.00%, 42.47% +/- 0.45% and 5.40% +/- 0.40%) of control group (P<0.05). But the cell proportion rate (36.37% +/- 0.40%) in the G1 phase in BQ group was significantly lower than that (52.13 +/- 0.75%) in control group (P<0.05). The expression level of p-Akt protein in BQ group was significantly higher than that in control group (P<0.05). The cell proliferation rate and the cell proportion in the S, G2 phase of LY294002 plus BQ group were 82.59% +/- 15.00%, 42.03% +/- 0.50% and 3.87% +/- 0.47%, respectively, which were significantly lower than those of BQ group (P<0.05). But the cell proportion rate (54.43% +/- 0.40%) in the G1 phase in LY294002 plus BQ group was significantly higher than that in BQ group (P<0.05).</p><p><b>CONCLUSION</b>The PI3K/Akt signal pathway may play an important role in the proliferation of HL-60 cells exposed to BQ.</p>

Association between SNPs in SOD2 and noise-induced hearing loss / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To observe the association between SNPs in SOD2 and noise-induced hearing loss in Chinese Han population.</p><p><b>METHODS</b>A case-control study was designed to study the effect of environmental risk factors on susceptibility to NIHL in 201 sensitive workers and 202 resistant workers. A questionnaire was designed to carry out an investigation, and an occupational health survey was used to identify the occupational risk factors. Genomic DNA was extracted from peripheral blood cells samples using standard procedures of Qiagen kit. SNPs were detected using standard procedures of TaqMan probe allele identification method.</p><p><b>RESULTS</b>In SOD2 gene, the C allele of rs4880 was a risk factor of NIHL, compared with the T allele, OR = 1.76, 95% CI 1.17 approximately 2.63, P = 0.006. CC and CT genotypes compared with TT were risk factors, crude OR = 2.24, 95% CI 1.43 approximately 3.50 and adjusted OR = 2.45, 95% CI 1.51 approximately 3.96, P < 0.001, respectively.</p><p><b>CONCLUSION</b>In Chinese Han population, SOD2 rs4880 SNP in the mitochondrial targeting sequence is associated with noise induced hearing loss.</p>

Effect of reactive oxygen species during the leukemogenic process associated with exposure to benzene / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the effect of reactive oxygen species (ROS) during the leukemogenic process associated with exposure to benzene.</p><p><b>METHODS</b>HL-60 was treated with 3 micromol/L benzoquinone (BQ). Generation of ROS in cells was measured by DCFH-DA method. For proliferation assays,cells were stained with alamar blue dye and counted.</p><p><b>RESULTS</b>ROS production and the proliferation of cell were all increased in BQ-treated cells (13.10 +/- 0.15, 185% +/- 30.00%) as compared with control cells (11.32 +/- 0.09, 100% +/- 0.00%) (P < 0.05); The addition of catalase just before BQ addition reduced ROS generation to basal levels and decreased the growth of cell (P < 0.05).</p><p><b>CONCLUSION</b>ROS may play an important role in the process of proliferation of HL-60 cells induced by BQ.</p>

Effect of environmental risk factors in occupational noise exposure to noise-induced hearing loss / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To observe the effect of environmental risk factors in occupational noise exposure on hearing loss and find out the susceptible population of noise induced hearing loss (NIHL).</p><p><b>METHODS</b>A case-control study was designed to study the effect of environmental risk factors on NIHL. 2400 workers exposed to 75 approximately 120 dB noises from an air conditioning factory in southern China served as the subjects. 202 workers were selected from 10% of population with the maximum hearing shift of the left ear to 3000 Hz noises as the NIHL susceptible group while 204 workers from 10% of population with the least hearing shift as the NIHL tolerant group. A questionnaire was designed to carry out an investigation, and an occupational health survey was used to identify the occupational risk factors which might affect the hearing system. The univariate analysis and multivariate analysis were used to observe the effect of environmental risk factors on NIHL.</p><p><b>RESULTS</b>The results of univariate analysis showed that smoking, alcohol drinking, organic solvent, heavy metal, heat, dust were significantly was associated with NIHL (P < 0.05). Multivariate analysis showed that only heat was associated with NIHL (P < 0.05), and OR value was 1.804 (95% CI: 1.101 approximately 2.958).</p><p><b>CONCLUSION</b>Exposure to heat may be a high risk factor of NIHL.</p>

Urinary S-phenylmercapturic acid variation in benzene exposed / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To observe the urinary S-phenylmercapturic acid (S-PMA) variation in the benzene dynamic exposed rat models and benzene exposed workers, and study the feasibility of use of urinary S-PMA as the biomarker in benzene exposed.</p><p><b>METHODS</b>In an animal model study, forty-eight adult Wistar rats were randomly divided into 4 groups: the control group, low-dose group, middle-dose group and high-dose group. The exposed groups were dynamically exposed for 28 days (4 periods) by benzene and the concentration was monitored. The urine was immediately collected after every exposure period and detected by the liquid chromatographic/mass spectrometry methods. In a cohort study, eighty benzene exposed workers in a ship-yard in Guangzhou were selected as the exposed subjects while forty healthy officers in the same shipyard who were not occupationally exposed to benzene were treated as the control. The urine was collected after work shift. The urinary S-PMA and the benzene in the workplace was treated as the rat model.</p><p><b>RESULTS</b>In the animal model study, the urinary S-PMA increased along with the environment benzene in every period and had significantly difference in the different exposed groups (P < 0.01 or P < 0.05), but did not change along with the exposed time course (P > 0.05). In the cohort study, the urinary S-PMA in the high-dose group [(27.2 +/- 7.9)microg/L] was significantly higher than the low-dose group [(13.6 +/- 3.4)microg/L] (P < 0.01). Otherwise, the background of urinary S-PMA was lower than 5microg/L in both workers and rat models.</p><p><b>CONCLUSION</b>The urinary S-PMA can be proposed as a sensitive biomarker of occupational benzene exposure.</p>